RT Journal Article
SR Electronic
T1 Single-cell immuno-mechanics: rapid viscoelastic changes are a hall-mark of early leukocyte activation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 851634
DO 10.1101/851634
A1 Alexandra Zak
A1 Sara Violeta Merino Cortés
A1 Anaïs Sadoun
A1 Avin Babataheri
A1 Stéphanie Dogniaux
A1 Sophie Dupré-Crochet
A1 Elodie Hudik
A1 Hai-Tao He
A1 Abdul I Barakat
A1 Yolanda R Carrasco
A1 Yannick Hamon
A1 Pierre-Henri Puech
A1 Claire Hivroz
A1 Oliver Nüsse
A1 Julien Husson
YR 2019
UL http://biorxiv.org/content/early/2019/11/22/851634.abstract
AB To accomplish their critical task of removing infected cells and fighting pathogens, leukocytes activate by forming specialized interfaces with other cells. Using an innovative micropipette rheometer, we show in three different cell types that when stimulated by microbeads mimicking target cells, leukocytes become up to ten times stiffer and more viscous. These mechanical changes initiate within seconds after contact and evolve rapidly over minutes. Remarkably, leukocyte elastic and viscous properties evolve in parallel, preserving a well-defined ratio that constitutes a mechanical signature specific to each cell type. The current results indicate that simultaneously tracking both elastic and viscous properties during an active cell process provides a new way to investigate cell mechanical processes. Our findings also suggest that dynamic immuno-mechanical measurements provide an identifier of leukocyte type and an indicator of the cell’s state of activation.