RT Journal Article
SR Electronic
T1 Cell-associated, Heparin-like Molecules Modulate the Ability of LDL to Regulate PCSK9 Uptake
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 329722
DO 10.1101/329722
A1 Adri M. Galvan
A1 John S. Chorba
YR 2018
UL http://biorxiv.org/content/early/2018/05/23/329722.abstract
AB Proprotein convertase subtilisin/kexin type 9 (PCSK9) targets the LDL receptor (LDLR) for degradation, increasing plasma LDL and, consequently, cardiovascular risk. Uptake of secreted PCSK9 is required for its predominant effect on the LDLR. LDL itself inhibits this uptake, though the mechanism by which it does so remains unclear. In this study, we investigated the relationship between LDL, the PCSK9:LDLR interaction, and PCSK9 uptake. We show that LDL inhibits binding of PCSK9 to the epidermal growth factor precursor homology domain A (EGF-A) domain of the LDLR in vitro more impressively than it inhibits PCSK9 uptake in cells. Furthermore, a cell-based factor responsive to heparin-targeting treatments can explain this difference, consistent with its identity as a cell surface heparan sulfate proteoglycan (HSPG), a known co-receptor for PCSK9. Furthermore, we show that the entire PCSK9 prodomain, but not truncated variants, rescues PCSK9 uptake in the presence of LDL, suggesting that PCSK9:LDL binding requires the entire prodomain. Additionally, we show that the gain-of-function (GOF) PCSK9 variant S127R has increased affinity for heparin-like molecules such as HSPGs, potentially explaining the biochemical basis for its phenotype. Overall, our findings suggest a model where PCSK9, LDL, and HSPGs all interact to regulate PCSK9 uptake into the hepatocyte.AbbreviationsPCSK9proprotein convertase subtilisin/kexin type 9LDLRlow density lipoprotein receptorEGF-Aepidermal growth factor precursor homology domain AHSPGheparan sulfate proteoglycanGOFgain-of-functionERendoplasmic reticulumNLucnanoluciferaseSDstandard deviationsd-LDLsmall dense LDLl-LDLlarge LDL