PT  - JOURNAL ARTICLE
AU  - Aybuke Alici-Garipcan
AU  - Burcu Özçimen
AU  - Ilke Suder
AU  - Volkan Ülker
AU  - Tamer T. Önder
AU  - Nesrin Özören
TI  - NLRP7 Plays A Functional Role in Regulating BMP4 Signaling During Differentiation of Patient-Derived Trophoblasts
AID  - 10.1101/850420
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 850420
4099  - http://biorxiv.org/content/early/2019/11/24/850420.short
4100  - http://biorxiv.org/content/early/2019/11/24/850420.full
AB  - Complete Hydatidiform Mole (HM) is a gestational trophoblastic disease resulting in hyper proliferation of trophoblast cells and absence of embryo development. Mutations in the primate specific-maternal effect gene NLRP7 are the major cause of familial recurrent complete HM. Here, we established an in vitro model of HM using NLRP7 deficient patient-specific induced pluripotent stem cells (iPSCs) derived trophoblasts. Using whole transcriptome profiling during trophoblast differentiation, we showed that NLRP7 deficiency results in precocious downregulation of pluripotency factors, activation of trophoblast lineage markers and promotes maturation of differentiated extraembryonic cell types such as syncytiotrophoblasts. Interestingly, we found that these phenotypes are dependent on BMP4 signaling and BMP pathway inhibition corrected the excessive trophoblast differentiation of patient derived iPSCs. Our human iPSC model of a genetic placental disease recapitulates aspects of trophoblast biology, highlights the broad utility of iPSC-derived trophoblasts for modeling human placental diseases and identifies NLRP7 as an essential modulator of key developmental cell fate regulators.