TY - JOUR T1 - Identification of a post-transcriptional regulatory element that responds to glucose in the African trypanosome JF - bioRxiv DO - 10.1101/327346 SP - 327346 AU - Yijian Qiu AU - Vijay Shankar AU - Rooksana E. Noorai AU - Nelson Yeung AU - Sarah Grace McAlpine AU - James Morris Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/05/24/327346.abstract N2 - The ability to adapt to varying nutrient availability in changing environments is critical for successful parasitism. The lifecycle stages of the African trypanosome, Trypanosoma brucei, that infect the host mammalian bloodstream utilize glucose exclusively for ATP production. The finding that trypanosomes also inhabit other tissues that frequently contain lower glucose concentrations suggests blood stage parasites may have to respond to a dynamic environment with changing nutrient availability in order to survive. However, little is known about how the parasites coordinate gene expression with nutrient availability. Through transcriptome analysis, we have found blood stage parasites deprived of glucose alter gene expression in a pattern similar to transcriptome changes triggered by other stresses. A surprisingly low concentration of glucose (<10 μM) was required to initiate the response. To further understand the dynamic regulation of gene expression that occurs in response to altered glucose availability in the environment, we have interrogated the 3’UTR of cytochrome c oxidase subunit VI, a known lifecycle stage regulated gene, and have identified a stem-loop structure that confers glucose-responsive regulation at the translational level. ER -