PT  - JOURNAL ARTICLE
AU  - Cristian A. Undurraga
AU  - Yunzi Gou
AU  - Pablo C. Sandoval
AU  - Viviana A. Nuñez
AU  - Miguel L. Allende
AU  - Bruce B. Riley
AU  - Pedro P. Hernández
AU  - Andres F. Sarrazin
TI  - Sox2 and Sox3 are essential for development and regeneration of the zebrafish lateral line
AID  - 10.1101/856088
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 856088
4099  - http://biorxiv.org/content/early/2019/11/26/856088.short
4100  - http://biorxiv.org/content/early/2019/11/26/856088.full
AB  - The recovery of injured or lost sensory neurons after trauma, disease or aging is a major scientific challenge. Upon hearing loss or balance disorder, regeneration of mechanosensory hair cells has been observed in fish, some amphibians and under special circumstances in birds, but is absent in adult mammals. In aquatic vertebrates, hair cells are not only present in the inner ear but also in neuromasts of the lateral line system. The zebrafish lateral line neuromast has an almost unlimited capacity to regenerate hair cells. This remarkable ability is possible due to the presence of neural stem/progenitor cells within neuromasts. In order to further characterize these stem cells, we use the expression of the neural progenitor markers Sox2 and Sox3, transgenic reporter lines, and morphological and topological analysis of the different cell types within the neuromast. We reveal new sub-populations of supporting cells, the sustentacular supporting cells and the neuromast stem cells. In addition, using loss-of-function and mutants of sox2 and sox3, we find that the combined activity of both genes is essential for lateral line development and regeneration. The capability of sox2/sox3 expressing stem cells to produce new hair cells, hair cell-precursors, and supporting cells after damage was analyzed in detail by time-lapse microscopy and immunofluorescence. We are able to provide evidence that sox2/3 expressing cells are the main contributors to the regenerated neuromast, and that their daughter cells are able to differentiate into most cell types of the neuromast.