RT Journal Article
SR Electronic
T1 Multiplexed Primer Extension Sequencing Enables High Precision Detection of Rare Splice Isoforms
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 331629
DO 10.1101/331629
A1 Hansen Xu
A1 Benjamin J. Fair
A1 Zach Dwyer
A1 Michael Gildea
A1 Jeffrey A. Pleiss
YR 2018
UL http://biorxiv.org/content/early/2018/05/25/331629.abstract
AB Targeted RNA-sequencing aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-sequencing experiments. Here we present a novel approach for targeted RNA-sequencing that uses complex pools of reverse transcription primers to enable sequencing enrichment at user-selected locations across the genome. We demonstrate this approach by targeting pre-mRNA splice junctions in S. cerevisiae, revealing high-precision detection of splice isoforms, including rare pre-mRNA splicing intermediates.