RT Journal Article SR Electronic T1 Refining the transcriptome of the human malaria parasite Plasmodium falciparum using amplification-free RNA-seq JF bioRxiv FD Cold Spring Harbor Laboratory SP 852038 DO 10.1101/852038 A1 Lia Chappell A1 Philipp Ross A1 Lindsey Orchard A1 Thomas D. Otto A1 Matthew Berriman A1 Julian C. Rayner A1 Manuel Llinás YR 2019 UL http://biorxiv.org/content/early/2019/11/29/852038.abstract AB Plasmodium parasites undergo several major developmental transitions during their complex lifecycle, which are enabled by precisely ordered gene expression programs. Transcriptomes from the 48-hour blood stages of the major human malaria parasite Plasmodium falciparum have been described using cDNA microarrays and RNA-seq, but these assays have not always performed well within non-coding regions, where the AT-content is often 90-95%. We developed a directional, amplification-free RNA-seq protocol (DAFT-seq) to reduce bias against AT-rich cDNA, which we have applied to three strains of P. falciparum (3D7, HB3 and IT). While strain-specific differences were detected, overall there is strong conservation between the transcriptional profiles. For the 3D7 reference strain, transcription was detected from 89% of the genome, with over 75% of the genome transcribed into mRNAs. These datasets allowed us to refine the 5’ and 3’ untranslated regions (UTRs), which can be variable, long (>1,000 nt), and often overlap those of adjacent transcripts. We also find that transcription from bidirectional promoters frequently results in non-coding, antisense transcripts. By capturing the 5’ ends of mRNAs, we reveal both constant and dynamic use of transcriptional start sites across the intraerythrocytic developmental cycle resulting in an updated view of the P. falciparum transcriptome.