TY - JOUR T1 - Primary mammary organoid model of lactation and involution JF - bioRxiv DO - 10.1101/859645 SP - 859645 AU - Jakub Sumbal AU - Aurelie Chiche AU - Elsa Charifou AU - Zuzana Koledova AU - Han Li Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/11/29/859645.abstract N2 - Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer.BOMbasal organoid medium;Csn2Casein2 - β-casein gene;ECMextracellular matrix;FGF2fibroblast growth factor 2;LMlactation medium;Mmpmatrix metalloproteinase;Wapwhey acidic protein. ER -