RT Journal Article
SR Electronic
T1 Cryo-OrbiSIMS for 3D molecular imaging of a bacterial biofilm in its native state
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 859967
DO 10.1101/859967
A1 Junting Zhang
A1 James Brown
A1 David Scurr
A1 Anwen Bullen
A1 Kirsty MacLellan-Gibson
A1 Paul Williams
A1 Morgan R. Alexander
A1 Kim R. Hardie
A1 Ian S. Gilmore
A1 Paulina D. Rakowska
YR 2019
UL http://biorxiv.org/content/early/2019/11/30/859967.abstract
AB We describe a method for label-free molecular imaging of biological materials, preserved in a native state, by using an OrbiSIMS instrument equipped with cryogenic sample handling and developing a high-pressure freezing protocol compatible with mass spectrometry. We studied the 3D distribution of quorum sensing signalling molecules, nucleobases and bacterial membrane molecules, in a mature Pseudomonas aeruginosa biofilm, with high spatial-resolution and high mass-resolution.