RT Journal Article
SR Electronic
T1 Bacterial diversity in deep-sea sediments under influence of asphalt seep at the São Paulo Plateau
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 753616
DO 10.1101/753616
A1 Luciano Lopes Queiroz
A1 Amanda Gonçalves Bendia
A1 Rubens Tadeu Delgado Duarte
A1 Diego Assis das Graças
A1 Artur Luiz da Costa da Silva
A1 Cristina Rossi Nakayama
A1 Paulo Yukio Sumida
A1 Andre O. S. Lima
A1 Yuriko Nagano
A1 Katsunori Fujikura
A1 Hiroshi Kitazato
A1 Vivian Helena Pellizari
YR 2019
UL http://biorxiv.org/content/early/2019/12/02/753616.abstract
AB Here we investigated the diversity of bacterial communities from deep-sea surface sediments under influence of asphalt seeps at the Sao Paulo Plateau using next-generation sequencing (NGS) method. Sampling was performed at North São Paulo Plateau using the human occupied vehicle Shinkai 6500 and her support vessel Yokosuka. The microbial diversity was studied at two surficial sediment layers (0-1 and 1-4 cm) of five samples collected in cores in water depths ranging from 2,456-2,728 m. Bacterial communities were studied through sequencing of 16S rRNA gene on the Ion Torrent platform and clustered in operational taxonomic units. We observed high diversity of bacterial sediment communities as previously described by other studies. When we considered community composition, the most abundant classes were Alphaprotebacteria (27.7%), Acidimicrobiia (20%), Gammaproteobacteria (11.3%) and Deltaproteobacteria (6.6%). Most abundant OTUs at family level were from two uncultured bacteria from Actinomarinales (5.95%) and Kiloniellaceae (3.17%). The unexpected high abundance of Alphaproteobacteria and Acidimicrobiia in our deep-sea microbial communities may be related to the presence of asphalt seep at North São Paulo Plateau, since these bacterial classes contain bacteria that possess the capability of metabolizing hydrocarbon compounds.