RT Journal Article SR Electronic T1 The Human RNA-Binding Proteome and Its Dynamics During Arsenite-Induced Translational Arrest JF bioRxiv FD Cold Spring Harbor Laboratory SP 329995 DO 10.1101/329995 A1 Trendel, Jakob A1 Schwarzl, Thomas A1 Prakash, Ananth A1 Bateman, Alex A1 Hentze, Matthias W. A1 Krijgsveld, Jeroen YR 2018 UL http://biorxiv.org/content/early/2018/05/30/329995.abstract AB Proteins and RNA functionally and physically intersect in multiple biological processes, however, currently no universal method is available to purify protein-RNA complexes. Here we introduce XRNAX, a method for the generic purification of protein-crosslinked RNA, and demonstrate its versatility to study the composition and dynamics of protein-RNA interactions by various transcriptomic and proteomic approaches. We show that XRNAX captures all RNA biotypes, and use this to characterize the sub-proteomes that interact with coding and non-coding RNAs (ncRNAs), and to identify hundreds of protein-RNA interfaces. Exploiting the quantitative nature of XRNAX, we observe drastic remodeling of the RNA-bound proteome during arsenite-induced stress, distinct from autophagy-induced changes in the total proteome. In addition, we combine XRNAX with CLIP-seq to validate the interaction of ncRNA with Lamin B and EXOSC2. Thus, XRNAX is a resourceful approach to study structural and compositional aspects of protein-RNA interactions to address fundamental questions in RNA-biology.