RT Journal Article
SR Electronic
T1 The in situ structures of mono-, di-, and tri-nucleosomes in human heterochromatin
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 334490
DO 10.1101/334490
A1 Shujun Cai
A1 Désirée Böck
A1 Martin Pilhofer
A1 Lu Gan
YR 2018
UL http://biorxiv.org/content/early/2018/05/30/334490.abstract
AB The in situ 3-D organization of chromatin at the nucleosome and oligonucleosome levels is unknown. Here we use cryo-electron tomography (cryo-ET) to determine the in situ structures of HeLa nucleosomes, which have canonical core structures and asymmetric, flexible linker DNA. Subtomogram remapping suggests that sequential nucleosomes in heterochromatin follow irregular paths at the oligonucleosome level. This basic principle of higher-order repressive chromatin folding is compatible with the conformational variability of the two linker DNAs at the single-nucleosome level.