RT Journal Article
SR Electronic
T1 Optogenetic manipulation of medullary neurons in the locust optic lobe
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 334003
DO 10.1101/334003
A1 Hongxia Wang
A1 Richard B. Dewell
A1 Markus U. Ehrengruber
A1 Eran Segev
A1 Jacob Reimer
A1 Michael L. Roukes
A1 Fabrizio Gabbiani
YR 2018
UL http://biorxiv.org/content/early/2018/05/31/334003.abstract
AB Locust is a widely used animal model for studying sensory processing and its relation to behavior. Due to the lack of genomic information, genetic tools to manipulate neural circuits in locusts are not yet available. We examined whether Semliki Forest virus is suitable to mediate exogenous gene expression in neurons of the locust optic lobe. We subcloned a channelrhodopsin variant and the yellow fluorescent protein Venus into a Semliki Forest virus vector and injected the virus into the optic lobe of locusts (Schistocerca americana). Fluorescence was observed in all injected optic lobes. Most neurons that expressed the recombinant proteins were located in the first two neuropils of the optic lobe, the lamina and medulla. Extracellular recordings demonstrated that laser illumination increased the firing rate of medullary neurons expressing channelrhodopsin. The optogenetic activation of the medullary neurons also triggered firing of a postsynaptic, looming-sensitive neuron, the Lobula Giant Movement Detector (LGMD). These results indicate that Semliki Forest virus is efficient at mediating transient exogenous gene expression and provides a tool to manipulate neural circuits in the locust nervous system and likely other insects.New and Noteworthy Using Semliki Forest virus, we efficiently delivered channelrhodopsin into neurons of the locust optic lobe. We demonstrate that laser illumination increases the firing of the medullary neurons expressing channelrhodopsin and of an identified postsynaptic target neuron, the LGMD neuron. This technique allows to manipulate the neuronal activity in locust neural circuits using optogenetics.Authors’ contributionsH.W., M.U.E., J.R., M.L.R. and F.G. designed the experiments; E.S. fabricated the laser probe; H.W. performed the experiments with the assistance of R.B.D and J.R.; H.W. carried out the data analysis with the assistance of R.B.D.; H.W. and F.G. co-wrote the paper.