RT Journal Article
SR Electronic
T1 Stress-induced alterations of norepinephrine release in the bed nucleus of the stria terminalis of mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 335653
DO 10.1101/335653
A1 Karl T. Schmidt
A1 Viren H. Makhijani
A1 Kristen M. Boyt
A1 Dipanwita Pati
A1 Melanie M. Pina
A1 Isabel M. Bravo
A1 Jason L. Locke
A1 Sara R. Jones
A1 Joyce Besheer
A1 Zoé A. McElligott
YR 2018
UL http://biorxiv.org/content/early/2018/05/31/335653.abstract
AB Stress can drive adaptive changes to maintain survival during threatening stimuli. Chronic stress exposure, however, may result in pathological adaptations. A key neurotransmitter involved in stress signaling is norepinephrine. Previous studies show that stress elevates norepinephrine levels in the bed nucleus of the stria terminalis (BNST), a critical node regulating anxiety and upstream of stress responses. Here, we use mice expressing channelrhodopsin in norepinephrine neurons to selectively activate terminals in the BNST, and measure norepinephrine release with fast-scan cyclic voltammetry. Mice exposed to a single restraint session show an identical norepinephrine release profile compared to that of unexposed mice. Mice experiencing five days of restraint stress, however, show elevated noradrenergic release across multiple stimulation parameters, and reduced sensitivity to the α2-adrenergic receptor antagonist idazoxan. These data are the first to examine norepinephrine release in the BNST to tonic and phasic stimulation frequencies, and confirm that repeated stress alters autoreceptor sensitivity.