RT Journal Article
SR Electronic
T1 Identification of key genes related to dexamethasone-resistance in acute lymphoblastic leukemia
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 337048
DO 10.1101/337048
A1 Qiuni Chen
A1 Shixin Chen
A1 Yuye Shi
A1 Shandong Tao
A1 Wei Chen
A1 Chunling Wang
A1 Liang Yu
YR 2018
UL http://biorxiv.org/content/early/2018/06/01/337048.abstract
AB Drug resistance is the main cause of poor chemotherapy response in acute leukemia. Despite the extensive use of dexamethasone(DEX) in the treatment of acute lymphoblastic leukemia for many years, the mechanisms of dexamethasone – resistance has not been fully understood. We choose GSE94302 from GEO database aiming to identify key genes that contribute to the DEX resistance in acute lymphoblastic leukemia. Differentially expressed gene(DEGs) are selected by using GEO2R tools. A total of 837 DEGs were picked out, including 472 up-regulated and 365 down-regulated DEGs. All the DEGs were underwent gene ontology(GO) analysis and Kyoto Encyclopedia of Gene and Genome(KEGG) pathway analysis. In addition, the DEGs-encoded protein-protein interaction (PPI) was screened by using Cytoscape and Search Tool for the Retrieval of Interacting Genes(STRING). Total 20 genes were found as key genes related to DEX resistance with high degree of connectivity, including CDK1, PCNA, CCNB1, MYC, KPNA2, AURKA, NDC80, HSPA4, KIF11, UBE2C, PIK3CG, CD44, CD19, STAT1, DDX41, LYN, BCR, CD48, JAK1 and ITGB1. They could be used as biomarkers to identify the DEX-resistant acute lymphoblastic leukemia.