@article {Martinez865790, author = {Viviana M Fajardo Martinez and Iris Feng and Bao Ying Chen and Cesar A Perez and Baochen Shi and Peter Clark and Rong Tian and Ching-Ling Lien and Matteo Pellegrini and Heather Christofk and Haruko Nakano and Atsushi Nakano}, title = {Glucose metabolism promotes neonatal heart regeneration}, elocation-id = {865790}, year = {2019}, doi = {10.1101/865790}, publisher = {Cold Spring Harbor Laboratory}, abstract = {The mammalian heart switches its main metabolic substrate from glucose to fatty acids shortly after birth. This metabolic switch coincides with the loss of regenerative capacity in the heart. However, it is unknown whether glucose metabolism itself regulates heart regeneration. Here, we report that glucose metabolism is a determinant of regenerative capacity in the neonatal mammalian heart. Cardiac-specific overexpression of Glut1, the embryonic form of constitutively active glucose transporter, resulted in an increase in glucose uptake and concomitant glycogen storage in postnatal heart. Upon cryoinjury, Glut1 transgenic hearts showed higher regenerative capacity with less fibrosis than non-transgenic control hearts. Interestingly, flow cytometry analysis revealed two distinct populations of ventricular cardiomyocytes: Tnnt2-high and Tnnt2-low cardiomyocytes, the latter of which showed significantly higher mitotic activity in response to high intracellular glucose in Glut1 transgenic hearts. Metabolic profiling shows that Glut1-transgenic hearts have a significant increase in the glucose metabolites upon injury, and inhibition of the nucleotide biosynthesis abrogated the regenerative advantage of high intra-cardiomyocyte glucose level. Our data suggest that the increased in glucose metabolism promotes cardiac regeneration in neonatal mouse heart.}, URL = {https://www.biorxiv.org/content/early/2019/12/09/865790}, eprint = {https://www.biorxiv.org/content/early/2019/12/09/865790.full.pdf}, journal = {bioRxiv} }