PT - JOURNAL ARTICLE AU - Joerg Doellinger AU - Andy Schneider AU - Timo Stark AU - Monika Ehling-Schulz AU - Peter Lasch TI - Evaluation of MALDI-ToF Mass Spectrometry for Rapid Detection of Cereulide from <em>Bacillus cereus</em> Cultures AID - 10.1101/869958 DP - 2019 Jan 01 TA - bioRxiv PG - 869958 4099 - http://biorxiv.org/content/early/2019/12/10/869958.short 4100 - http://biorxiv.org/content/early/2019/12/10/869958.full AB - Bacillus cereus plays an often unrecognized role in food borne diseases. Food poisoning caused by this pathogen is manifested by either diarrhea or emesis. While different enterotoxins have been linked to the diarrheal type of B. cereus infections, the emetic toxin cereulide is responsible for the second type. Due to the relatively high prevalence of cereulide associated food poisoning, methods for simple and reliable detection of cereulide producing strains are of utmost importance. Currently, liquid-chromatography coupled to mass spectrometry (LC-MS) is used for sensitive, specific and quantitative cereulide detection, but this technique requires specialized LC-MS equipment, which is often not available in microbiology routine diagnostic laboratories.The last decade has witnessed the advent of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF MS) as a simple, rapid and cost-efficient technique for identification of microbial pathogens in routine diagnostics. Just recently, two different studies reported on the application of MALDI-ToF MS for either the differentiation of emetic and non-emetic strains of B. cereus or for direct detection of cereulide from bacterial colony smears. However, no method evaluation and optimization was performed in frame of these studies. Thus, additional investigations on the selectivity and sensitivity of MALDI-TOF MS for cereulide detection are needed before implementation of this method in routine diagnostics can be considered. These aspects prompted us to investigate open or controversial issues and to systematically test sample preparation methods, commonly used for microbial identification for their suitability to detect the emetic toxin directly from bacteria.Based on our experimental findings we propose a MALDI-ToF MS workflow that allows identification of B. cereus and sensitive detection of cereulide in parallel, using standard, linear-mode MALDI-ToF MS equipment. The experimental protocol is based on the well-established ethanol/formic acid extraction method and offers, if required, possibilities for further characterization by more sophisticated LC-MS-based methods. In summary, the ease of use and the achieved level of analytical sensitivity as well as the wide-spread availability of standard MALDI-ToF MS equipment in clinical microbiological laboratories provides a promising tool to improve and to facilitate routine diagnostics of B. cereus associated food intoxications.ACNacetonitrileFAformic acidHCCAα-cyano-4-hydroxycinnamic acidLCliquid-chromatographyLDIlaser desorption/ionizationLODlimit of detectionMALDI-ToFmatrix-assisted laser desorption/ionization - time–of–flightMSmass spectrometryNRPSnon-ribosomal peptide synthetaseSIDAstable isotope dilution analysisSNRsignal-to-noise ratioThThomsonTFAtrifluoroacetic acid