RT Journal Article
SR Electronic
T1 Activation of macrophages by lysophosphatidic acid through the lysophosphatidic acid receptor 1 as a novel mechanism in multiple sclerosis pathogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 870980
DO 10.1101/870980
A1 Jennifer Fransson
A1 Ana Isabel Gómez
A1 Jesús Romero-Imbroda
A1 Oscar Fernández
A1 Laura Leyva
A1 Fernando Rodriguez de Fonseca
A1 Jerold Chun
A1 Celine Louapre
A1 Anne Baron Van-Evercooren
A1 Violetta Zujovic
A1 Guillermo Estivill-Torrús
A1 Beatriz García-Díaz
YR 2019
UL http://biorxiv.org/content/early/2019/12/11/870980.abstract
AB Multiple sclerosis (MS) is a neuro-inflammatory disease for which the pathogenesis remains largely unclear. Lysophosphatidic acid (LPA) is an endogenous phospholipid that is involved in multiple immune cell functions and is dysregulated in MS. Its receptor LPA1 is expressed in macrophages and regulates their activation, which is of interest due to the role of macrophage activation in MS in both destruction and repair.In this study, we studied the viable Malaga variant of LPA1-null mutation as well as pharmaceutical inhibition of LPA1 in mice with experimental autoimmune encephalomyelitis (EAE), a model of MS. LPA1 expression was also analyzed in both wild-type EAE mice and MS patient immune cells. The effect of LPA and LPA1 on macrophage activation was studied in human monocyte-derived macrophages.We show that lack of LPA1 activity induces a milder clinical course in EAE, and that Lpar1 expression in peripheral blood mononuclear cells (PBMCs) correlates with onset of relapses and severity in wild-type EAE mice. We see the same over-expression in PBMCs from MS patients during relapse compared to progressive forms of the disease, and in monocyte-derived macrophages after exposure to pro-inflammatory stimuli. In addition, LPA induced a pro-inflammatory-like response in macrophages through LPA1, providing a plausible way in which LPA and LPA1 dysregulation can lead to the inflammation seen in MS.These data show a new mechanism of LPA signaling in the pathogenesis of MS, prompting further research into its use as a therapeutic target biomarker.