PT  - JOURNAL ARTICLE
AU  - Song, Chanwoo
AU  - Lee, Gu-Haeng
AU  - Oh, JunTaek
AU  - Lee, Moosung
AU  - Shin, Seungwoo
AU  - Lee, SangYun
AU  - Nam, Yoonkey
AU  - Park, YongKeun
TI  - Measurements of 3-D morphology of individual red blood cells upon localized photothermal effects using gold nanorods
AID  - 10.1101/2019.12.11.872218
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 2019.12.11.872218
4099  - http://biorxiv.org/content/early/2019/12/12/2019.12.11.872218.short
4100  - http://biorxiv.org/content/early/2019/12/12/2019.12.11.872218.full
AB  - We investigated the in-situ photothermal response of human red blood cells (RBCs) by combining photothermal heat generation and 3D quantitative phase imaging techniques. Gold-nanorod-coated substrates were excited using near-infrared light to generate local heat to RBCs, and response was measured by imaging 3-D refractive index tomograms of cells under various near infrared (NIR) excitation conditions. On photothermal treatment, cell morphology changed from discoid to crescent shapes, cell volume and dry mass decreased, and hemoglobin concentration increased. We also investigated the irreversible deformation of RBCs when multiple intense excitation shocks are applied. These results provide a new understanding of thermodynamic aspects of cell biology and hematology.