RT Journal Article
SR Electronic
T1 Measurements of 3-D morphology of individual red blood cells upon localized photothermal effects using gold nanorods
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2019.12.11.872218
DO 10.1101/2019.12.11.872218
A1 Song, Chanwoo
A1 Lee, Gu-Haeng
A1 Oh, JunTaek
A1 Lee, Moosung
A1 Shin, Seungwoo
A1 Lee, SangYun
A1 Nam, Yoonkey
A1 Park, YongKeun
YR 2019
UL http://biorxiv.org/content/early/2019/12/12/2019.12.11.872218.abstract
AB We investigated the in-situ photothermal response of human red blood cells (RBCs) by combining photothermal heat generation and 3D quantitative phase imaging techniques. Gold-nanorod-coated substrates were excited using near-infrared light to generate local heat to RBCs, and response was measured by imaging 3-D refractive index tomograms of cells under various near infrared (NIR) excitation conditions. On photothermal treatment, cell morphology changed from discoid to crescent shapes, cell volume and dry mass decreased, and hemoglobin concentration increased. We also investigated the irreversible deformation of RBCs when multiple intense excitation shocks are applied. These results provide a new understanding of thermodynamic aspects of cell biology and hematology.