TY - JOUR T1 - Multi-photon attenuation-compensated light-sheet fluorescence microscopy JF - bioRxiv DO - 10.1101/2019.12.12.873877 SP - 2019.12.12.873877 AU - Madhu Veettikazhy AU - Jonathan Nylk AU - Federico Gasparoli AU - Adrià Escobet-Montalbán AU - Anders Kragh Hansen AU - Dominik Marti AU - Peter Eskil Andersen AU - Kishan Dholakia Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/12/12/2019.12.12.873877.abstract N2 - Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an increase in depth penetration for imaging. Here we show this promising approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, furthermore, be achieved in a facile manner utilizing a graded neutral density filter, circumventing the need for complex beam shaping apparatus. A “gold standard” system utilizing a spatial light modulator for beam shaping is used to benchmark our implementation. The approach will open up enhanced depth penetration in light-sheet imaging to a wide range of end users. ER -