PT  - JOURNAL ARTICLE
AU  - Jatin Panwar
AU  - Rahul Roy
TI  - Integrated fusible alloy microelectrodes based microfluidic impedance cytometry for cell-in-droplet quantification
AID  - 10.1101/278069
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 278069
4099  - http://biorxiv.org/content/early/2018/06/07/278069.short
4100  - http://biorxiv.org/content/early/2018/06/07/278069.full
AB  - Microfluidic impedance cytometry (MIC) provides a non-optical and label-free method for single cell analysis. However, the cleanroom intensive infrastructure required for MIC electrode fabrication limits its implementation. As an alternate technique that enables rapid prototyping, we fabricated Field’s metal ‘in-contact’ (icFM) coplanar microelectrodes in multilayer elastomer devices with a single photolithography step and characterised them for microfluidic impedance cytometry. Our icFM microelectrodes matched performance of the conventional platinum electrodes in the detection of single human erythrocytes and water-in-oil droplets in a feedback-controlled suction-flow MIC setup. Finally, to facilitate droplet based single cell analysis, we demonstrate detection and quantification of single erythrocytes entrapped in water-in-oil droplets.