TY - JOUR T1 - A Multi-Niche Microvascularized Human Bone-Marrow-on-a-Chip JF - bioRxiv DO - 10.1101/2019.12.15.876813 SP - 2019.12.15.876813 AU - Michael R. Nelson AU - Delta Ghoshal AU - Joscelyn C. Mejías AU - David Frey Rubio AU - Emily Keith AU - Krishnendu Roy Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/12/15/2019.12.15.876813.abstract N2 - The human bone marrow (hBM) is a complex organ critical for hematopoietic and immune homeostasis, and where many cancers metastasize. Yet, understanding the fundamental biology of the hBM in health and diseases remain difficult due to complexity of studying or manipulating the BM in humans. Accurate in vitro models of the hBM microenvironment are critical to further our understanding of the BM niche and advancing new clinical interventions. Although, in vitro culture models that recapitulate some key components of the BM niche have been reported, there are no examples of a fully human, in vitro, organoid platform that incorporates the various niches of the hBM - specifically the endosteal, central marrow, and perivascular niches – thus limiting their physiological relevance. Here we report an hBM-on-a-chip that incorporates these three niches in a single micro-physiological device. Osteogenic differentiation of hMSCs produced robust mineralization on the PDMS surface (“bone layer”) and subsequent seeding of endothelial cells and hMSCs in a hydrogel network (“central marrow”) created an interconnected vascular network (“perivascular niche”) on top. We show that this multi-niche hBM accurately mimics the ECM composition, allows hematopoietic progenitor cell proliferation and migration, and is affected by radiation. A key finding is that the endosteal niche significantly contributes to hBM physiology. Taken together, this multi-niche micro-physiological system opens up new opportunities in hBM research and therapeutics development, and can be used to better understand hBM physiology, normal and impaired hematopoiesis, and hBM pathologies, including cancer metastasis, multiple myelomas, and BM failures. ER -