RT Journal Article SR Electronic T1 Kinetic sculpting of the seven stripes of the Drosophila even-skipped gene JF bioRxiv FD Cold Spring Harbor Laboratory SP 335901 DO 10.1101/335901 A1 Augusto Berrocal A1 Nicholas Lammers A1 Hernan G. Garcia A1 Michael B. Eisen YR 2018 UL http://biorxiv.org/content/early/2018/06/11/335901.abstract AB We used live imaging to visualize the transcriptional dynamics of the Drosophila melanogaster even-skipped gene at single-cell and high temporal resolution as its iconic seven stripe expression pattern forms. We developed a rigorous quantitative framework for analyzing and visualizing these data, that allows us to infer the temporal dynamics of the transcriptional state of roughly 3,000 nuclei, and characterize how the process varies over time and space. We show that despite being created by the largely independent activity of five discrete enhancers, the seven eve stripes are sculpted by the same basic kinetic phenomena: the suppression of bursting outside of stripes, a coupled increase of burst frequency and burst duration within stripes over time and across stripes from their margins to their centers, and the exchange of active nuclei from the posterior to anterior flanks to reposition stripes over time.