PT - JOURNAL ARTICLE AU - Egle Cekanaviciute AU - Anne-Katrin Pröbstel AU - Anna Thomann AU - Tessel F. Runia AU - Patrizia Casaccia AU - Ilana Katz Sand AU - Elizabeth Crabtree AU - Sneha Singh AU - John Morrissey AU - Patrick Barba AU - Refujia Gomez AU - Rob Knight AU - Sarkis K. Mazmanian AU - Jennifer Graves AU - Bruce A.C. Cree AU - Scott S. Zamvil AU - Sergio E. Baranzini TI - Multiple sclerosis-associated changes in the composition and immune functions of spore-forming bacteria AID - 10.1101/343558 DP - 2018 Jan 01 TA - bioRxiv PG - 343558 4099 - http://biorxiv.org/content/early/2018/06/11/343558.short 4100 - http://biorxiv.org/content/early/2018/06/11/343558.full AB - Multiple sclerosis (MS) is an autoimmune disease of the central nervous system characterized by adaptive and innate immune system dysregulation. Recent work has revealed moderate alteration of gut microbial communities in subjects with MS and in experimental, induced models. However, a mechanistic understanding linking the observed changes in the microbiota and the presence of the disease is still missing. Chloroform-resistant, spore-forming bacteria have been shown to exhibit immunomodulatory properties in vitro and in vivo, but they have not yet been characterized in the context of human disease. This study addresses the community composition and immune function of this bacterial fraction in MS. We identify MS-associated spore-forming taxa and show that their presence correlates with impaired differentiation of IL-10 secreting, regulatory T lymphocytes in-vitro. Colonization of antibiotic-treated mice with spore-forming bacteria allowed us to identify some bacterial taxa favoring IL-10+ lymphocyte differentiation and others inducing differentiation of pro-inflammatory, IFNγ+ T lymphocytes. However, when fed into antibiotic-treated mice, both MS and control derived spore-forming bacteria were able to induce immunoregulatory responses.Our analysis also identified Akkermansia muciniphila as a key organism that may interact either directly or indirectly with spore-forming bacteria to exacerbate the inflammatory effects of MS-associated gut microbiota. Thus, changes in the spore-forming fraction may influence T lymphocyte-mediated inflammation in MS. This experimental approach of isolating a subset of microbiota based on its functional characteristics may be useful to investigate other microbial fractions at greater depth.Importance Despite the rapid emergence of microbiome related studies in human diseases, few go beyond a simple description of relative taxa levels in a select group of patients. Our study integrates computational analysis with in vitro and in vivo exploration of inflammatory properties of both complete microbial communities and individual taxa, revealing novel functional associations. We specifically show that while small differences exist between the microbiomes of MS patients and healthy subjects, these differences are exacerbated in the chloroform resistant fraction. We further demonstrate that, when purified from MS patients, this fraction is associated with impaired immunomodulatory responses in vitro.