PT  - JOURNAL ARTICLE
AU  - Katarzyna O. Rojek
AU  - Joanna Krzemień
AU  - Hubert Doleżyczek
AU  - Paweł M. Boguszewski
AU  - Leszek Kaczmarek
AU  - Witold Konopka
AU  - Marcin Rylski
AU  - Jacek Jaworski
AU  - Lars Holmgren
AU  - Tomasz J. Prószyński
TI  - Amot regulates neuronal dendritic tree through Yap1
AID  - 10.1101/345264
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 345264
4099  - http://biorxiv.org/content/early/2018/06/12/345264.short
4100  - http://biorxiv.org/content/early/2018/06/12/345264.full
AB  - The Amot-Yap1 complex plays a major role in the regulation of cell contact inhibition, cellular polarity and growth. However, the function of Angiomotin (Amot) and Hippo pathway transcription co-activator Yap1 in the central nervous system remains unclear. In this study, we demonstrate that Amot is a critical mediator of dendritic morphogenesis in cultured hippocampal cells and Purkinje cells in the brain. Amot function in developing hippocampal neurons depends on interactions with Yap1, which is also indispensable for dendrite growth and arborization in vitro. Conditional deletion of Amot or Yap1 in neurons leads to impaired morphogenesis of Purkinje cell dendritic trees, decreased cerebellar size, and causes defects in locomotor coordination of mutant animals. Thus, our studies identified Amot and Yap1 as novel regulators of dendritic tree morphogenesis.