RT Journal Article
SR Electronic
T1 A synthetic non-histone substrate provides insight into substrate targeting by the Gcn5 HAT and sirtuin HDACs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 345637
DO 10.1101/345637
A1 Anthony Rössl
A1 Mong-Shang Lin
A1 Michael Downey
YR 2018
UL http://biorxiv.org/content/early/2018/06/12/345637.abstract
AB Gcn5 and sirtuins are highly conserved HAT and HDAC enzymes that were first characterised as regulators of gene expression. Although histone tails are important substrates of these enzymes, these proteins also target many non-histone substrates that participate in diverse biological processes. The mechanisms used by these enzymes to choose their non-histone substrates is unclear. In this work, we use a unique synthetic biology approach in S. cerevisiae to demonstrate that a shared target sequence can act as a determinant of substrate selection for Gcn5 and sirtuins. We also exploit this system to define specific subunits of the Gcn5-containing ADA complex as regulators of non-histone acetylations proteome-wide.