TY - JOUR T1 - Genome-wide rules of nucleosome phasing JF - bioRxiv DO - 10.1101/093666 SP - 093666 AU - Sandro Baldi AU - Dhawal S. Jain AU - Lisa Harpprecht AU - Angelika Zabel AU - Marion Scheibe AU - Falk Butter AU - Tobias Straub AU - Peter B. Becker Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/06/13/093666.abstract N2 - Regular successions of positioned nucleosomes – phased nucleosome arrays (PNAs) – are predominantly known from transcriptional start sites (TSS). It is unclear whether PNAs occur elsewhere in the genome. To generate a comprehensive inventory of PNAs for Drosophila, we applied spectral analysis to nucleosome maps and identified thousands of PNAs throughout the genome. About half of them are not near TSS and strongly enriched for a novel sequence motif. Through genome-wide reconstitution of physiological chromatin in Drosophila embryo extracts we uncovered the molecular basis of PNA formation. We identified Phaser, an unstudied zinc finger protein that positions nucleosomes flanking the new motif. It also revealed how the global activity of the chromatin remodeler CHRAC/ACF, together with local barrier elements, generates islands of regular phasing throughout the genome. Our work demonstrates the potential of chromatin assembly by embryo extracts as a powerful tool to reconstitute chromatin features on a global scale in vitro. ER -