RT Journal Article
SR Electronic
T1 Optimization and functionalization of red-shifted rhodamine dyes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2019.12.20.881227
DO 10.1101/2019.12.20.881227
A1 Jonathan B. Grimm
A1 Ariana N. Tkachuk
A1 Heejun Choi
A1 Boaz Mohar
A1 Natalie Falco
A1 Ronak Patel
A1 Jennifer Lippincott-Schwartz
A1 Timothy A. Brown
A1 Luke D. Lavis
YR 2019
UL http://biorxiv.org/content/early/2019/12/20/2019.12.20.881227.abstract
AB Expanding the palette of fluorescent dyes is vital for pushing the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups.