@article {Vasiliauskait{\'e}-Brooks348219, author = {Ieva Vasiliauskait{\'e}-Brooks and Robert D. Healey and Pascal Rochaix and R{\'e}my Sounier and Claire Grison and Thierry Waltrich-Augusto and Mathieu Fortier and Fran{\c c}ois Hoh and Essa M. Saied and Christoph Arenz and Shibom Basu and C{\'e}dric Leyrat and S{\'e}bastien Granier}, title = {Structure of a human intramembrane ceramidase explains enzymatic dysfunction found in leukodystrophy}, elocation-id = {348219}, year = {2018}, doi = {10.1101/348219}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Alkaline ceramidases (ACERs) are a class of poorly understood transmembrane enzymes controlling the homeostasis of ceramides. They are implicated in human pathophysiology, including progressive leukodystrophy, colon cancer as well as acute myeloid leukemia. We report here the crystal structure of the human ACER type 3 (ACER3). Together with computational studies, the structure reveals that ACER3 is an intramembrane enzyme with a seven transmembrane domain architecture and a catalytic Zn2+ binding site in its core, similar to adiponectin receptors. Interestingly, we uncover a Ca2+ binding site physically and functionally connected to the Zn2+ providing a structural explanation for the known regulatory role of Ca2+ on ACER3 enzymatic activity and for the loss of function in E33G-ACER3 mutant found in leukodystrophic patients.}, URL = {https://www.biorxiv.org/content/early/2018/06/15/348219}, eprint = {https://www.biorxiv.org/content/early/2018/06/15/348219.full.pdf}, journal = {bioRxiv} }