PT - JOURNAL ARTICLE AU - Devendra K. Biswal AU - Tanmoy Roychowdhury AU - Priyatama Pandey AU - Veena Tandon TI - <em>De novo</em> genome and transcriptome analyses provide insights into the biology of the trematode human parasite <em>Fasciolopsis buski</em> AID - 10.1101/354456 DP - 2018 Jan 01 TA - bioRxiv PG - 354456 4099 - http://biorxiv.org/content/early/2018/06/23/354456.short 4100 - http://biorxiv.org/content/early/2018/06/23/354456.full AB - Many trematode parasites cause infection in humans and are thought to be a major public health problem. Their ecological diversity in different regions provides challenging questions on evolution of these organisms. In this report, we perform transcriptome analysis of the giant intestinal fluke, Fasciolopsis buski, using next generation sequencing technology. Short read sequences derived from polyA containing RNA of this organism were assembled into 30677 unigenes that led to the annotation of 12380 genes. Annotation of the assembled transcripts enabled insight into processes and pathways in the intestinal fluke, such as RNAi pathway and energy metabolism. The expressed kinome of the organism was characterized by identifying all protein kinases. We have also carried out whole genome sequencing and used the sequences to confirm absence of some of the genes, not observed in transcriptome data, such as genes involved in fatty acid biosynthetic pathway. Transcriptome data also helped us to identify some of the expressed transposable elements. Though many Long Interspersed elements (LINEs) were identified, only two Short Interspersed Elements (SINEs) were visible. Overall transcriptome and draft genome analysis of F. buski helped us to characterize some its important biological characteristics and provided enormous resources for development of a suitable diagnostic system and anti-parasitic therapeutic molecules.