PT - JOURNAL ARTICLE AU - Rhys Parry AU - Sassan Asgari TI - Aedes anphevirus (AeAV): an insect-specific virus distributed worldwide in <em>Aedes aegypti</em> mosquitoes that has complex interplays with <em>Wolbachia</em> and dengue virus infection in cells AID - 10.1101/335067 DP - 2018 Jan 01 TA - bioRxiv PG - 335067 4099 - http://biorxiv.org/content/early/2018/06/25/335067.short 4100 - http://biorxiv.org/content/early/2018/06/25/335067.full AB - Insect specific viruses (ISVs) of the yellow fever mosquito Aedes aegypti have been demonstrated to modulate transmission of arboviruses such as dengue virus (DENV) and West Nile virus by the mosquito. The diversity and composition of the virome of Ae. aegypti, however, remains poorly understood. In this study, we characterised Aedes anphevirus (AeAV), a negative-sense RNA virus from the order Mononegavirales. AeAV identified from Aedes cell lines were infectious to both Ae. aegypti and Aedes albopictus cells, but not to three mammalian cell lines. To understand the incidence and genetic diversity of AeAV, we assembled 17 coding-complete and two partial genomes of AeAV from available RNA-Seq data. AeAV appears to transmit vertically and be present in laboratory colonies, wild-caught mosquitoes and cell lines worldwide. Phylogenetic analysis of AeAV strains indicates that as the Ae. aegypti mosquito has expanded into the Americas and Asia-Pacific, AeAV has evolved into monophyletic African, American and Asia-Pacific lineages. The endosymbiotic bacterium Wolbachia pipientis restricts positive-sense RNA viruses in Ae. aegypti. Re-analysis of a small RNA library of Ae. aegypti cells co-infected with AeAV and Wolbachia produces an abundant RNAi response consistent with persistent virus replication. We found Wolbachia enhances replication of AeAV when compared to a tetracycline cleared cell line, and AeAV modestly reduces DENV replication in vitro. The results from our study improve understanding of the diversity and evolution of the virome of Ae. aegypti and adds to previous evidence that shows Wolbachia does not restrict a range of negative strand RNA viruses.