RT Journal Article
SR Electronic
T1 Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 356329
DO 10.1101/356329
A1 Jan. P. Gerlach
A1 Jessie A. G. van Buggenum
A1 Sabine E.J. Tanis
A1 Mark Hogeweg
A1 Branco M. H. Heuts
A1 Mauro J. Muraro
A1 Lisa Elze
A1 Francesca Rivello
A1 Agata Rakszewska
A1 Alexander van Oudenaarden
A1 Wilhelm T. S. Huck
A1 Hendrik G. Stunnenberg
A1 Klaas W. Mulder
YR 2018
UL http://biorxiv.org/content/early/2018/06/27/356329.abstract
AB Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogenenous cellular responses to environmental signals at the mRNA and phospho-proteome level.