RT Journal Article
SR Electronic
T1 microRNA profiling of mouse cortical progenitors and neurons reveals miR-486-5p as a novel regulator of neurogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2019.12.27.889170
DO 10.1101/2019.12.27.889170
A1 Martina Dori
A1 Daniel Cavalli
A1 Mathias Lesche
A1 Simone Massalini
A1 Leila Haj Abdullah Alieh
A1 Beatriz Cardoso de Toledo
A1 Sharof Khudayberdiev
A1 Gerhard Schratt
A1 Andreas Dahl
A1 Federico Calegari
YR 2019
UL http://biorxiv.org/content/early/2019/12/28/2019.12.27.889170.abstract
AB MicroRNAs (miRNAs) are short (∼22 nt) single-stranded non-coding RNAs that regulate gene expression at the post-transcriptional level. Over the past years, many studies have extensively characterized the involvement of miRNA-mediated regulation in neurogenesis and brain development. However, a comprehensive catalog of cortical miRNAs cell-specifically expressed in progenitor types of the developing mammalian cortex is still missing. Overcoming this limitation, here we exploited a double reporter mouse line previously validated by our group to allow the identification of the transcriptional signature to neurogenic commitment and provide the field with the complete atlas of miRNAs expression in proliferating neural stem cells, neurogenic progenitors and newborn neurons during corticogenesis. By extending the currently known list of miRNAs expressed in the mouse brain by over two fold, our study highlights the power of cell type-specific analyses for the detection of transcripts that would otherwise be diluted out when studying bulk tissues. We further exploited our data by predicting putative novel miRNAs and validated the power of our approach by providing novel evidence for the involvement of miR-486 as a novel player in brain development.