PT  - JOURNAL ARTICLE
AU  - Shin, Dongju
AU  - Lee, Wookjae
AU  - Lee, Ji Hyun
AU  - Bang, Duhee
TI  - Multiplexed single-cell RNA-seq via transient barcoding for drug screening
AID  - 10.1101/359851
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 359851
4099  - http://biorxiv.org/content/early/2018/06/30/359851.short
4100  - http://biorxiv.org/content/early/2018/06/30/359851.full
AB  - To simultaneously analyze multiple samples of various conditions with scRNA-seq, we developed a universal sample barcoding method through transient transfection of SBOs. A 48-plex drug treatment experiment of pooled samples analyzed by a single run of Drop-Seq revealed a unique transcriptome response for each drug and target-specific gene expression signatures at the single-cell level. Our cost-effective method is widely applicable for single-cell profiling of multiple experimental conditions.