RT Journal Article
SR Electronic
T1 Multiplexed single-cell RNA-seq via transient barcoding for drug screening
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 359851
DO 10.1101/359851
A1 Dongju Shin
A1 Wookjae Lee
A1 Ji Hyun Lee
A1 Duhee Bang
YR 2018
UL http://biorxiv.org/content/early/2018/06/30/359851.abstract
AB To simultaneously analyze multiple samples of various conditions with scRNA-seq, we developed a universal sample barcoding method through transient transfection of SBOs. A 48-plex drug treatment experiment of pooled samples analyzed by a single run of Drop-Seq revealed a unique transcriptome response for each drug and target-specific gene expression signatures at the single-cell level. Our cost-effective method is widely applicable for single-cell profiling of multiple experimental conditions.