%0 Journal Article %A Jifeng Hu %A Varunyu Khamviwath %A Hans G. Othmer %T A Stochastic Model for Actin Waves in Eukaryotic Cells %D 2019 %R 10.1101/2019.12.31.892034 %J bioRxiv %P 2019.12.31.892034 %X A stochastic model of spontaneous actin wave formation in eukaryotic cells that includes positive feedback between the actin network and filament nucleating factors on the membrane is developed and analyzed. Simulation results show that the model can produce a variety of actin network behavior depending on the conditions. Actin spots of diameter about 0.5 µm can be formed and persist for tens of seconds at low actin concentrations, and these spots may either shrink and die or grow and develop into fully-developed propagating waves. The model correctly captures the vertical profile of actin waves along line scans through wave fronts, as well as the separation between the region enclosed by circular actin waves and the external area. Our results show how the complicated actin behavior depends on the amounts and state of various membrane molecules.Author Summary Locomotion of eukaryotic cells is a complex process that involves the spatio-temporal control and integration of a number of sub-processes, including the transduction of chemical or mechanical signals from the environment, local and global modification of the cytoskeleton, and translation of the intra- and extracellular signals into a mechanical response. In view of the complexity of the processes, understanding how force generation and mechanical interactions with the surroundings are controlled in space and time to produce cell-level movement is a major challenge. Recent experimental work has shown that a variety of actin waves propagate within cells, both under normal conditions and during re-building of the cytoskeleton following its disruption. Controlled disruption and re-building of the actin network has led to new insights into the key components involved in actin waves, and here we develop a stochastic model that can qualitatively and quantitatively describe the dynamical behavior of such waves. %U https://www.biorxiv.org/content/biorxiv/early/2019/12/31/2019.12.31.892034.full.pdf