PT  - JOURNAL ARTICLE
AU  - Miles R Armstrong
AU  - Jack Vossen
AU  - Tze Yin Lim
AU  - Ronald C B Hutten
AU  - Jianfei Xu
AU  - Shona M Strachan
AU  - Brian Harrower
AU  - Nicolas Champouret
AU  - Eleanor M Gilroy
AU  - Ingo Hein
TI  - Tracking disease resistance deployment in potato breeding by enrichment sequencing
AID  - 10.1101/360644
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 360644
4099  - http://biorxiv.org/content/early/2018/07/02/360644.short
4100  - http://biorxiv.org/content/early/2018/07/02/360644.full
AB  - Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.