RT Journal Article SR Electronic T1 Tracking disease resistance deployment in potato breeding by enrichment sequencing JF bioRxiv FD Cold Spring Harbor Laboratory SP 360644 DO 10.1101/360644 A1 Miles R Armstrong A1 Jack Vossen A1 Tze Yin Lim A1 Ronald C B Hutten A1 Jianfei Xu A1 Shona M Strachan A1 Brian Harrower A1 Nicolas Champouret A1 Eleanor M Gilroy A1 Ingo Hein YR 2018 UL http://biorxiv.org/content/early/2018/07/02/360644.abstract AB Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.