%0 Journal Article %A Anabelle Planques %A Vanessa Oliveira Moreira %A Chantal Dubreuil %A Alain Prochiantz %A Ariel A Di Nardo %T OTX2 signals from the choroid plexus to regulate adult neurogenesis %D 2018 %R 10.1101/243659 %J bioRxiv %P 243659 %X Proliferation and migration during adult neurogenesis are regulated by a microenvironment of signaling molecules originating from local vasculature, from cerebrospinal fluid produced by the choroid plexus, and from local supporting cells including astrocytes. Here, we focus on the function of OTX2 homeoprotein transcription factor in the mouse adult ventricular-subventricular zone (V-SVZ) which generates olfactory bulb neurons. We find that OTX2 secreted by choroid plexus is transferred to supporting cells of the V-SVZ and rostral migratory stream. Deletion of Otx2 in choroid plexus reduces the number of olfactory bulb newborn neurons and modifies extracellular matrix components produced by astrocytes. Similar results are obtained by expressing secreted single-chain antibodies to sequester OTX2 in the cerebrospinal fluid, demonstrating the importance of non-cell autonomous OTX2 in adult neurogenesis and suggesting a pivotal role for astrocytes. Thus, we reveal a multi-level and non-cell autonomous role of a homeoprotein and reinforce the choroid plexus as a key niche compartment affecting adult neurogenesis. %U https://www.biorxiv.org/content/biorxiv/early/2018/07/03/243659.full.pdf