PT  - JOURNAL ARTICLE
AU  - Christopher Amourda
AU  - Timothy E. Saunders
TI  - The mirtron miR-1010 functions in concert with its host gene SKIP to maintain synaptic homeostasis
AID  - 10.1101/353888
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 353888
4099  - http://biorxiv.org/content/early/2018/07/03/353888.short
4100  - http://biorxiv.org/content/early/2018/07/03/353888.full
AB  - Mirtrons are non-canonical miRNAs arising by splicing and debranching from short introns. A plethora of introns have been inferred by computational analyses as potential mirtrons. Yet, few have been experimentally validated and their functions, particularly in relation to their host genes, remain poorly understood. Here, we found that larvae lacking the mirtron miR-1010 are unable to grow properly and pupariate. We show that miR-1010 downregulates nAcRβ2. Increase of cortical nAcRβ2 mediated by neural activity elevates the level of intracellular Ca2+, which in turn activates CaMKII and, further downstream, the transcription factor Adf-1. We reveal that Adf-1 initiates the expression of SKIP, the host gene of miR-1010. Preventing synaptic potentials from overshooting their optimal range requires both SKIP to temper synaptic potentials (incoherent feedforward loop) and miR-1010 to reduce nAcRβ2 mRNA levels (negative feedback loop). Our results demonstrate how a mirtron, in coordination with its host gene, contributes to maintaining homeostasis.