RT Journal Article
SR Electronic
T1 Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 362517
DO 10.1101/362517
A1 Daniel Sage
A1 Thanh-An Pham
A1 Hazen Babcock
A1 Tomas Lukes
A1 Thomas Pengo
A1 Ramraj Velmurugan
A1 Alex Herbert
A1 Anurag Agrawal
A1 Silvia Colabrese
A1 Ann Wheeler
A1 Anna Archetti
A1 Bernd Rieger
A1 Raimund Ober
A1 Guy M. Hagen
A1 Jean-Baptiste Sibarita
A1 Jonas Ries
A1 Ricardo Henriques
A1 Michael Unser
A1 Seamus Holden
YR 2018
UL http://biorxiv.org/content/early/2018/07/04/362517.abstract
AB With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designed, in a large community effort, a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities – 2D, astigmatic 3D, biplane 3D, and double helix 3D – and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.