TY - JOUR T1 - Three dimensional parallelized RESOLFT nanoscopy for volumetric live cell imaging JF - bioRxiv DO - 10.1101/2020.01.09.898510 SP - 2020.01.09.898510 AU - Andreas Bodén AU - Francesca Pennacchietti AU - Ilaria Testa Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/01/09/2020.01.09.898510.abstract N2 - The volumetric architecture of organelles and molecules inside cells can only be investigated with microscopes featuring sufficiently high resolving power in all three spatial dimensions. Current methods suffer from severe limitations when applied to live cell imaging such as long recording times and/or photobleaching. By introducing a novel optical scheme to switch reversibly switchable fluorescent molecules, we demonstrate volumetric nanoscopy of living cells with resolution below 100 nm in 3D, large field of view and minimal illumination intensities (W-kW/cm2). ER -