TY - JOUR T1 - Proteomics Dissection of Cardiac Protein Profiles of Humans and Model Organisms JF - bioRxiv DO - 10.1101/2020.01.08.897595 SP - 2020.01.08.897595 AU - Nora Linscheid AU - Alberto Santos AU - Pi Camilla Poulsen AU - Robert W. Mills AU - Christian Stolte AU - Ulrike Leurs AU - Johan Z. Ye AU - Kirstine Calloe AU - Morten B. Thomsen AU - Bo H. Bentzen AU - Pia R. Lundegaard AU - Morten S. Olesen AU - Lars J. Jensen AU - Jesper V. Olsen AU - Alicia Lundby Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/01/09/2020.01.08.897595.abstract N2 - The study of human cardiac pathologies often relies on research conducted in model organisms to gain molecular insight into disease and to develop novel treatment strategies; however, translating findings from model organisms back to human can present a significant challenge, in part due to a lack of knowledge about the differences across species in cardiac protein abundances and their interactions. Here we set out to bridge this knowledge gap by presenting a global analysis of cardiac protein expression profiles in humans and commonly used model organisms. Using quantitative mass spectrometry-based proteomics, we measured the abundance of ~7,000 proteins in samples from the separate chambers of human, pig, horse, rat, mouse and zebrafish hearts. This knowledgebase of cardiac protein signatures is accessible through an online database at: atlas.cardiacproteomics.com. Quantitative comparison of the protein profiles support the pig as model organism of choice for arrhythmogenic right ventricular cardiomyopathy whereas comparison of profiles from the two-chambered zebrafish heart suggests a better resemblance to the right side of mammalian hearts. This proteomics resource facilitates translational prospect of cardiac studies from model organisms to humans by enabling direct comparison of disease-linked protein networks across species. ER -