RT Journal Article
SR Electronic
T1 Proteomics Dissection of Cardiac Protein Profiles of Humans and Model Organisms
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.08.897595
DO 10.1101/2020.01.08.897595
A1 Nora Linscheid
A1 Alberto Santos
A1 Pi Camilla Poulsen
A1 Robert W. Mills
A1 Christian Stolte
A1 Ulrike Leurs
A1 Johan Z. Ye
A1 Kirstine Calloe
A1 Morten B. Thomsen
A1 Bo H. Bentzen
A1 Pia R. Lundegaard
A1 Morten S. Olesen
A1 Lars J. Jensen
A1 Jesper V. Olsen
A1 Alicia Lundby
YR 2020
UL http://biorxiv.org/content/early/2020/01/09/2020.01.08.897595.abstract
AB The study of human cardiac pathologies often relies on research conducted in model organisms to gain molecular insight into disease and to develop novel treatment strategies; however, translating findings from model organisms back to human can present a significant challenge, in part due to a lack of knowledge about the differences across species in cardiac protein abundances and their interactions. Here we set out to bridge this knowledge gap by presenting a global analysis of cardiac protein expression profiles in humans and commonly used model organisms. Using quantitative mass spectrometry-based proteomics, we measured the abundance of ~7,000 proteins in samples from the separate chambers of human, pig, horse, rat, mouse and zebrafish hearts. This knowledgebase of cardiac protein signatures is accessible through an online database at: atlas.cardiacproteomics.com. Quantitative comparison of the protein profiles support the pig as model organism of choice for arrhythmogenic right ventricular cardiomyopathy whereas comparison of profiles from the two-chambered zebrafish heart suggests a better resemblance to the right side of mammalian hearts. This proteomics resource facilitates translational prospect of cardiac studies from model organisms to humans by enabling direct comparison of disease-linked protein networks across species.