PT  - JOURNAL ARTICLE
AU  - Claire Deo
AU  - Ahmed S. Abdelfattah
AU  - Hersh K. Bhargava
AU  - Adam J. Berro
AU  - Natalie Falco
AU  - Benjamien Moeyaert
AU  - Mariam Chupanova
AU  - Luke D. Lavis
AU  - Eric R. Schreiter
TI  - Bright and tunable far-red chemigenetic indicators
AID  - 10.1101/2020.01.08.898783
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.01.08.898783
4099  - http://biorxiv.org/content/early/2020/01/09/2020.01.08.898783.short
4100  - http://biorxiv.org/content/early/2020/01/09/2020.01.08.898783.full
AB  - Functional imaging using fluorescent indicators has revolutionized biology but additional sensor scaffolds are needed to access properties such as bright, far-red emission. We introduce a new platform for ‘chemigenetic’ fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. This approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in neurons.