RT Journal Article
SR Electronic
T1 Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.01.09.900241
DO 10.1101/2020.01.09.900241
A1 Noriki Fujimoto
A1 Yuliang He
A1 Marco D’Addio
A1 Carlotta Tacconi
A1 Michael Detmar
A1 Lothar C. Dieterich
YR 2020
UL http://biorxiv.org/content/early/2020/01/09/2020.01.09.900241.abstract
AB Lymph nodes (LNs) are highly organized secondary lymphoid organs that mediate adaptive immune responses to antigens delivered via afferent lymphatic vessels. Lymphatic endothelial cells (LECs) line intranodal lymphatic sinuses and organize lymph and antigen distribution. LECs also directly regulate T cells, mediating peripheral tolerance to self-antigens, and play a major role in many diseases including cancer metastasis. However, little is known about the phenotypic and functional heterogeneity of LN LECs. Using single-cell RNA sequencing, we comprehensively defined the transcriptome of LECs in murine skin-draining LNs, and identified new markers and functions of distinct LEC subpopulations. We found that LECs residing in the subcapsular sinus have an unanticipated function in scavenging of modified LDL and also identified a specific cortical LEC subtype implicated in rapid lymphocyte egress from LNs. Our data provide new insights into the diversity of LECs in murine lymph nodes and a rich resource for future studies into the regulation of immune responses by lymph node LECs.