TY - JOUR T1 - Structure of the ciliary axoneme at nanometer resolution reconstructed by TYGRESS JF - bioRxiv DO - 10.1101/363317 SP - 363317 AU - Kangkang Song AU - Zhiguo Shang AU - Xiaofeng Fu AU - Xiaochu Lou AU - Nikolaus Grigorieff AU - Daniela Nicastro Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/07/06/363317.abstract N2 - The resolution of subtomogram averages calculated from cryo-electron tomograms (cryo-ET) of crowded cellular environments is often limited due to signal loss in, and misalignment of the subtomograms. In contrast, single-particle cryo-electron microcopy (SP-cryo-EM) routinely reaches near-atomic resolution of isolated complexes. We developed a novel hybrid-method called “TomographY-Guided 3D REconstruction of Subcellular Structures” (TYGRESS) that combines cryo-ET with SP-cryo-EM to achieve close-to-nanometer resolution of complexes inside crowded environments. Using TYGRESS, we determined the native 3D structures of the intact ciliary axoneme with up to 12 Å resolution. These results reveal many structures and details that were not visible by cryo-ET. TYGRESS is generally applicable to cellular complexes that are amenable to subtomogram averaging, bringing us a step closer to (pseudo-)atomic models of cells.One Sentence Summary A hybrid cryo-electron microscopy method reveals subcellular structures at unprecedented resolution. ER -