TY - JOUR T1 - Enabling high-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing JF - bioRxiv DO - 10.1101/645903 SP - 645903 AU - Søren M. Karst AU - Ryan M. Ziels AU - Rasmus H. Kirkegaard AU - Emil A. Sørensen AU - Daniel McDonald AU - Qiyun Zhu AU - Rob Knight AU - Mads Albertsen Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/01/11/645903.abstract N2 - High-throughput amplicon sequencing of large genomic regions remains challenging for short-read technologies. Here, we report a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies or Pacific Biosciences CCS sequencing, yielding high accuracy single-molecule consensus sequences of large genomic regions. Our approach generates amplicon and genomic sequences of >10,000 bp in length with a mean error-rate of 0.0049-0.0006% and chimera rate <0.022%. ER -