TY - JOUR T1 - Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells JF - bioRxiv DO - 10.1101/160374 SP - 160374 AU - Anna O. Chertkova AU - Marieke Mastop AU - Marten Postma AU - Nikki van Bommel AU - Sanne van der Niet AU - Kevin L. Batenburg AU - Linda Joosen AU - Theodorus W.J. Gadella, Jr. AU - Yasushi Okada AU - Joachim Goedhart Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/01/13/160374.abstract N2 - To increase our understanding of the inner working of cells, there is a need for specific markers to identify biomolecules, cellular structures and compartments. One type of markers comprises genetically encoded fluorescent probes that are linked with protein domains, peptides and/or signal sequences. These markers are encoded on a plasmid and they allow straightforward, convenient labeling of cultured mammalian cells by introducing the plasmid into the cells. Ideally, the fluorescent marker combines favorable spectroscopic properties (brightness, photostability) with specific labeling of the structure or compartment of interest. Here, we report our ongoing efforts to generate robust and bright genetically encoded fluorescent markers for highlighting structures and compartments in living cells. The plasmids are distributed by addgene: https://www.addgene.org/browse/article/28189953/ ER -